Figure 3

Impact of γc cytokines on the production of transcription factors and cytotoxic effector proteins in recently-activated CD8 ⁺ T cells. Purified naïve OT-I CD8⁺ T cells (1x10⁶/ml) were stimulated with peptide-pulsed APCs (6x10⁶/ml). After 48 hours, OT-I T cells were harvested, re-purified and re-cultured (5x10⁵/ml) in the presence of either plain media or media supplemented with IL-2, IL-4, IL-7, IL-15 or IL-21 (100 ng/ml). After 24 hours, cells were harvested and the analyzed by intracellular cytokine staining and flow cytometry. A, B) Production of transcription factors associated with T cell fate determination. C, D) Production of effector proteins associated with cytotoxic CD8⁺ T cell function. Data are generated from 3 biological replicates, histograms reflect a single representative biological replicate and the bar graphs depict the mean +/−SD (n = 3). Unless otherwise noted, significance reflects the difference between the treatment group and the media only control. *P < 0.05, *P < 0.01, ***P < 0.001.