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Figure 1 | Journal for ImmunoTherapy of Cancer

Figure 1

From: Early transduction produces highly functional chimeric antigen receptor-modified virus-specific T-cells with central memory markers: a Production Assistant for Cell Therapy (PACT) translational application

Figure 1

Optimization of early transduction of EBVSTs with a retroviral vector encoding GFP. PBMCs were stimulated with autologous EBV-LCLs with or without IL-4 and IL-7. Two days later, the cells were transduced with a high titer retroviral vector encoding GFP. The transgene (GFP) expression by EBVSTs transduced (A) on day 2 (ET) or (B) on day 19 (LT) was determined by FACS analysis after the number of stimulations indicated. (C) Show the total cell numbers of Day 2 (ET) transduced cells in the presence or absence of IL-4 and IL-7 over time. Data represent the mean +/− sd of 2 donors.

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