Fig. 1

Indium-111 labeling does not impact T-cell viability nor diminish antigen specific cytokine secretory function. a Cell viability of unlabeled controls (white), 48uCi (grey), and 490uCi labeled T-cells (black) in media only, with IL-2, or CD3/CD28 bead stimulation after culture for 4 and 24 h. b Levels of IFN-g (pg/ml) secreted in 48 h culture supernatants of unlabeled controls (white), 48uCi (grey), and 490uCi (black) labeled T-cells cultured with media only, IL-2, after CMV antigen stimulation, or CD3/CD28 stimulation. Columns and bars represent the mean of duplicates (± SE) for each condition for both donors