Fig. 20

(Abstract P2). a Immunoblot of moesin knockout (KO or MsnKO) in multiple organs of mice. b Representative enlarged spleen size and increased cell number as observed in MsnKO mice. Data are reported as the mean ± SEM; ***P <0.001 by Student’s t-test. n = 6 per group. c and d Flow cytometry of inflammatory cytokines, TNF-α and IL-4 (c), and IFN-γ and IL-17A (d) produced by CD4+ T cells from spleens of WT and MsnKO mice after PMA-Ionomycin stimulation for 4 h. Data are reported as the mean ± SEM; *P <0.05 and ***P <0.001 by Student’s t-test. WT n = 3, MsnKO n = 4. e and f Flow cytometry analysis and absolute number of pTregs (Foxp3+Heliolo) in the small intestine lamina propria (sLP) (e) and colon lamina propria (cLP) (f) of 10-12 weeks old mice. Data are reported as the mean ± SEM; *P <0.05, **P<0.01 and ***P <0.001 by Student’s t-test. n = 4 per group, MsnKO sLP n = 3. g and h Flow cytometry analysis and absolute number of pTregs (Foxp3+Helioslo) in the spleen (g) and peripheral blood (h) of 10-12 weeks old mice. Data are reported as the mean ± SEM; ns, not significant; *P <0.05 and **P <0.01 by Student’s t-test. n = 4 per group