Fig. 23

(Abstract P2). a-c EL4 LAF (EL4) cells transduced with lentiviral scrambled vector (Scram) or moesin shRNA (MsnKD), stimulated with TGF-β (5 ng/ml) and analyzed for intracellular pSmad2/3 by flow cytometry at the times indicated. Data are representative of the mean ± SD of at least three independent experiments. *P <0.05 and **P <0.01 by Student’s t-test. (d and e) Analyses of phospho-Smad2/3 (pSmad2/3) by flow cytometry after 3-day cultures (d) and pSmad3 and total Smad3 by immunoblotting (e) stimulated at the indicated times in WT and MsnKO iTregs. Data are representative of at least two independent experiments.