Fig. 35

(Abstract P47). Treatment of allo-BMT recipients with a VIP antagonist induces a CD8+ donor T cell-dependent GvL response associated with down-regulation of PD-L1 on donor pDC and expansion of oligoclonal donor CD8+ T cells. A: Survival curves and GVHD clinical scores for B10.BR-->B6 allo-BMT harboring C1498 leukemia cells. Donor T cell subsets were depleted of specific populations by MACS prior to transplantation as shown. B: Expression of PD-L1 on donor pDC in B6-->B10.BR transplants. C: TCR Vbeta and J gene segments present in donorCD8+ T cells (left), CD8+ T cells from mice with GVHD (middle) and Cd8+ T cells from mice with GvL (right). D: Lack of correspondence between TCR Vbeta and J genes sequenced in mice with GvHD and mice with GVL