Fig. 40

(Abstract P67). a Circulating % NKT cells are reduced in BC patients. Peripheral blood mononuclear cells (PBMC) were collected from healthy donors (HD) and breast cancer patients (BC), stained for NKT TCR and analyzed by flow cytometry. b NKT cell function correlates total T cell function in BC patients. PBMCs from BC patients were stimulated with CD1D-Ig aAPC loaded with a-GalCer to specifically activate NKT cells or anti-CD3/CD28 to activate T cells. Media serves as a negative control and PMA/ionomycin was the positive control. After 4 hours, RNA was extracted and qPCR was performed to assess IFN-g and 18S. Fold induction was calculated relative to the control