Fig. 66

(Abstract P180). a TCR transgenic PMEL CD8+ T cells were stimulated for 7 days with Db-gp100 signal 1 only particles with increasing molar doses of anti-CD28 signal 2 (s2) only particles (i.e. 2X = 2:1 molar excess signal 2). aAPC refers to traditional signal 1 and 2 aAPCs. Cells were incubated in the presence or absence of a magnetic field for the first three days, and cell counts were taken on day 7. b PMEL CD8+ T cells were stimulated for 7 days with signal 1 (s1) only particles, signal 1 and 2 on separate particles (s1 + s2), or signal 1 and 2 on the same particle (s1/2 aAPC). After 7 days, T cell mediated killing of target B16-F10 cells was measured (*p<0.05 by two-way ANOVA)