Fig. 55 (abstract P339).

Intratumoral injection of MVA∆E3L or MVA is effective in a B16-F10 bilateral implantation model. B16-F10 bilateral tumor implantation model was used to assess the anti-tumor efficacy of MVA∆E3L vs MVA. Briefly, B16-F10 melanoma cells were implanted intradermally to the left and right flanks of C57B/6 mice (5 x 10e5 to the right flank and 1 x 10e5 to the left flank). 8 days after tumor implantation, the larger tumors on the right flank were intratumorally injected with 2 x 10e7 pfu of MVA or an equivalent amount of MVAΔE3L. The tumor sizes were measured and the tumors were re-injected twice a week. The survival of mice was monitored. Graphs of injected (a, c, e) and non-injected (b, d, f) tumor volume plotted against time (days) after PBS, MVA, or MVAΔE3L injection respectively. g is a Kaplan-Meier survival curve of tumor-bearing mice (B16-F10 cells) injected with PBS (filled circles), MVA (filled squares), or MVAΔE3L (filled triangles). ****, p < 0.0001 (MVA∆E3L vs. PBS group); ***, p < 0.001 (MVA vs. PBS group)