Fig. 76 (abstract P382).

Transcriptome analysis in NK cells. Purified NK cells from HD were isolated and transcriptome analysis was performed under two different conditions a) IL-2 stimulated NK cells that showed evidence of priming through epigenetic modification indicators (HIST1H1C, HIST1H4I, ZNF286B, HIST2H2BE); b IL-2/IL-12 stimulated NK cells that up-regulated mRNA encoding cytokines (IFN-γ, IL-1ß, IL-6 and TNF) and chemokines (CCL3, CXCL1, CXCL3) consistent with an activation phenotype. This approach will be useful to show which pathways and genes are modulated during NK cell exhaustion when the analysis will be repeated in NK cells from MD as represented in Fig. 75