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Fig. 82 (abstract P383). | Journal for ImmunoTherapy of Cancer

Fig. 82 (abstract P383).

From: 31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016): part two

Fig. 82 (abstract P383).

MAFs Suppress PD-L1 Expression and Promote Melanoma Progression in a Wnt5a-dependent Manner. a Generation of MAFs from resected BRAF(V600E)PTEN−/− melanoma tissues. b Transwell cultures of MAFs and BRAF(V600E)PTEN−/− melanoma cells followed by PD-L1 flow cytometry. c Transwell culture of MAFs and BRAF(V600E)PTEN−/− melanoma cells +/− TGFBi followed by Wnt5a Western blot analysis. d Primary tumor growth of co-transplanted MAFs with control BRAF(V600E)PTEN−/− melanoma cells (NTC) vs Wnt5a-silenced BRAF(V600E)PTEN−/− melanoma cells (Wnt5aKD). e qrt-PCR and immunofluorescence of PD-L1 expression by BRAF(V600E)PTEN−/−NTC melanomas vs BRAF(V600E)PTEN−/−Wnt5aKD melanomas

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