Clinical and immunologic correlates of response to PD-1 blockade in a patient with metastatic renal medullary carcinoma
© The Author(s). 2017
Received: 1 November 2016
Accepted: 15 December 2016
Published: 17 January 2017
Renal medullary carcinoma (RMC) is a rare kidney tumor that occurs in adolescent and young adults, typically in association with sickle cell trait. RMC exhibits rapid disease progression, frequent metastases at diagnosis, and dismal clinical outcomes. Currently available therapies, including cisplatin-based combination chemotherapy, multi-tyrosine kinase, and mTOR inhibitor strategies demonstrate either transient responses or minimal activity. Therefore, further molecular characterization and additional treatment strategies are urgently needed in this aggressive disease. The role of immune system surveillance and responsiveness to anti-PD-1 therapies in RMC are completely unexplored.
A 29 year old male with sickle cell trait presented with painless hematuria that ultimately resulted in a diagnosis of RMC. He underwent total nephrectomy and adjuvant cytotoxic chemotherapy with carboplatin, gemcitabine, paclitaxel, and bevacizumab. As is common in this aggressive form of kidney cancer he recurred with biopsy proven lymph node metastasis. He was started on checkpoint inhibitor therapy with nivolumab that inhibits program cell death protein 1 (PD-1), and on his first follow-up imaging he was found to have a partial response that on subsequent scans ultimately resulted in a complete response lasting greater than nine months. In this report, we present a patient with metastatic RMC who exhibited a clinical response to nivolumab, as well as the genetic and immunologic correlates of the pre-treatment tumor. Provocatively, robust immune infiltrate and expression of immune checkpoints were observed, despite the presence of a low mutation burden.
Here, we report the first case of immune microenvironment profiling and response to anti-PD-1 in a patient with RMC to our knowledge. This case suggests that anti-PD-1 based therapies may have clinical activity in RMC.
KeywordsNivolumab PD-1 Renal medullary carcinoma Renal cell carcinoma
Renal medullary carcinoma (RMC) is a rare cancer often referred to as the “seventh sickle cell nephropathy” . The typical patient with RMC is an adolescent or young adult (<40 years old) with sickle cell trait, although other hemoglobinopathies and patients with sickle cell disease have also been encountered . Presenting symptoms include gross hematuria, flank pain, weight loss, fatigue, and often symptoms of metastatic disease. Imaging at diagnosis demonstrates mass lesions centered in the renal medulla, frequently with associated satellite lesions and metastatic spread. The majority of patients present with advanced disease at diagnosis, and RMC has a rapid natural progression of disease with a mean life span with nephrectomy of 16 months [3, 4].
The biology of RMC is only recently being elucidated. The most common genetic event that has been observed is loss of SMARCB1/INI1. This was first recognized in 2008 based on loss of protein expression , and genomic analysis revealed allelic loss and inactivation . More recently, balanced translocations in the tumor suppressor SMARCB1 have been observed as a cause for this loss . Notably there has been a lack of additional genetic alterations and overall these tumors display relative genome stability . The functional implications of losing this key Swi/Snf complex member remain uncertain. Importantly, the immunologic characteristics of this tumor type have not been thoroughly examined, although one preliminary study suggests that many RMC tumors express PD-L1 . In particular, the profile of tumor infiltrating lymphocytes, immune checkpoint expression, and response to anti-PD-1/PD-L1 have not been described.
Due the rarity of RMC and its rapid clinical progression, therapeutic choices are informed by case reports and small patient series rather than randomized clinical trials. Several reports have shown that patients with RMC can transiently respond to combination platinum-based cytotoxic chemotherapy, but experience little benefit when treated with VEGF or mTOR inhibitors, agents that are conventionally used for clear cell renal cell carcinoma [9–13]. Thus, better therapies for this disease are urgently needed. Here, we report a clinical response of a patient with RMC to PD-1 inhibition, and survey the immunologic characteristics of the pre-treatment tumor.
A 29 year old previously healthy African American male with sickle cell trait sought evaluation for new onset hematuria. Computed tomography (CT) imaging showed a lesion at the pole of his left kidney and aortocaval lymphadenopathy; a biopsy was consistent with renal medullary carcinoma. He underwent an open left partial nephrectomy with retroperitoneal lymph node dissection. Surgical pathology showed a 2.2 cm tumor with positive margins, Fuhrman nuclear grade 4, involvement of the renal pelvis, and lymphatic invasion with an associated mass of lymph nodes measuring 3 cm in diameter (AJCC stage III – pT1aN1Mx). Next generation sequencing identified only two genomic alterations of known significance, including the loss of SMARCB1 as a homozyogous copy number deletion, and a frame shift mutation in ARID2 (H1495fs*8).
He then underwent flexible bronchoscopy and biopsy of a right paratracheal lymph node that confirmed disease recurrence. He was initiated on treatment with nivolumab, a PD-1 inhibitor, at 3 mg/kg every two weeks. On his initial CT scan, he was found to have an excellent partial response. At the time of this report, the patient has continued to tolerate nivolumab well with only mild fatigue, and has worked full-time throughout treatment. Now nine months after initiation of PD-1 inhibition, his CT scan showed complete response (Fig. 1b) almost 32 months from the time of his initial diagnosis.
Here, we report the first case of immune microenvironment profiling and response to anti-PD-1 in a patient with RMC to our knowledge. RMC is a rare subtype of kidney cancer most commonly diagnosed at advanced stage with a poor overall survival measured in months. We present a unique case of a patient diagnosed with locally advanced disease who underwent surgical resection followed by adjuvant chemotherapy with subsequent rapid disease progression. Radiation to the site of progression was locally effective but he developed simultaneous metastatic spread to multiple mediastinal lymph nodes thereafter. He initiated treatment with the PD-1 inhibitor nivolumab resulting in complete response which is ongoing 9 months into therapy. To our knowledge, this is the first characterization of immune profiling of RMC, and the first case of response to anti-PD-1 in this disease state.
Nivolumab, a monoclonal antibody inhibiting PD-1, was recently approved by the FDA as second line treatment for patients with advanced renal cell carcinoma (RCC). PD-1 is a cell surface signaling receptor found on chronically stimulated T cells which dampens their response and provides negative feedback inhibition. Nivolumab produced a 25% overall response rate compared to 5% with the mTOR inhibitor everolimus in patients with metastatic RCC, thus becoming one of few approved drugs to extend overall survival in this disease (in this case by 5.4 months) . Patients on this and other immunotherapy clinical trials, however, have primarily included the clear cell subtype; RMC is a rare enough entity to not have been studied in this arena to date. There are now numerous ongoing clinical trials using checkpoint inhibitors targeting PD-1 and its ligand PD-L1 in new settings including as upfront treatment, in combination with other checkpoint inhibitors, and in a rapidly expanding range of malignancies.
Numerous efforts to identify markers that may predict response to anti-PD-1 are ongoing, including total numbers of somatic mutations and markers of immune infiltrate (particularly CD8, PD-1, PD-L1, and CTLA-4). High overall mutational burden in various tumor types including colon cancer with mismatch repair deficiency, non-small cell lung cancer, urothelial bladder cancer, and melanoma has also been suggested as a predictive biomarker of response to checkpoint inhibition [15–18]. By contrast, whole exome sequencing and next generation gene panel approaches have found that RMC tumors are genomically quite stable. One could speculate that epigenetic modification, stemming from loss of SMARCB1, leads to presentation of neoantigens triggering responses to PD-1 inhibition [19–21]. Mutations in ARID2 like that found in our patient, have been idenitified to occur in up to 10% of papillary renal cell carcinoma . Interestingly, ARID2 is also a member of the SWI/SNF chromatin remodeling complex which suggests that loss of function of this complex is important in the pathophysiology of RMC and could lead to enhanced presentation of neoantigens. Notably, our patient was found to have a vigorous immune infiltrate including CD4+ and CD8+ T cells and high levels of immune checkpoint expression, including PD-L1, CTLA-4, and PD-1, which suggest pre-existing immune recognition, and have also been linked to immune therapy responses [23–25]. By contrast, the patient was found to have a very low calculated mutation burden using targeted next generation sequencing. Thus, this study suggests alternative paradigms of immune responsiveness beyond mutational load.
In an adolescent and young adult disease such as RMC where median overall survival is 16 months and current treatment options are suboptimal, additional biologic insights and novel therapeutics are urgently needed. This case report suggests a potential role for PD-1/PD-L1 inhibition in RMC. Further investigation in RMC and other rare RCC subtypes is necessary to determine the benefit of immune checkpoint inhibitors in patients with various subtypes of kidney cancer.
American joint comission on cancer
Area under the curve
Cytotoxic T-lymphocyte associated protein 4
Food and drug administration
Mammalian target of rapamycin
Progammed cell death 1 protein
Programmed death ligand-1
Positron emission tomography
Renal cell carcinoma
Renal medullary carcinoma
SWI/SNF Related matrix associated actin dependent regulator of chromatin
This work was supported by funding from the National Institutes of Health (K23 CA204726, D.J.; K24CA172355, W.K.R.), the Merck-Cancer Research Institute Irvington fellowship (K.B.), and the Brock Endowment fellowship (K.B.).
Availability of data and materials
Data sharing is not applicable to this article as no datasets were generated or analyzed during the current study.
KB collected and analyzed the data and wrote the manuscript; JK. and JB. helped perform immunofluorescent staining of patient samples, IP and PJ performed patient care. WKR and DJ provided expertise and wrote and edited the manuscript. All authors reviewed the manuscript. All authors read and approved the final manuscript.
DBJ is on the advisory board for BMS and Genoptix and receives research funding from Incyte. JYK and JB are employees of Genoptix. No other authors report conflicts of interest.
Consent for publication
JITC journal consent was signed by the patient and is available upon request.
Ethics approval and consent to participate
A waiver of consent was obtained to investigate the clinical and molecular markers of response to immune therapy by the Vanderbilt Institutional Review Board.
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- Davis Jr CJ, Mostofi FK, Sesterhenn IA. Renal medullary carcinoma. The seventh sickle cell nephropathy. Am J Surg Pathol. 1995;19:1–11.View ArticlePubMedGoogle Scholar
- Alvarez O, Rodriguez MM, Jordan L, Sarnaik S. Renal medullary carcinoma and sickle cell trait: a systematic review. Pediatr Blood Cancer. 2015;62:1694–9.View ArticlePubMedGoogle Scholar
- Figenshau RS, Basler JW, Ritter JH, Siegel CL, Simon JA, et al. Renal medullary carcinoma. J Urol. 1998;159:711–3.View ArticlePubMedGoogle Scholar
- Shah AY, Karam JA, Malouf GG, Rao P, Lim ZD, et al. Management and Outcomes of Patients with Renal Medullary Carcinoma: A Multi-Center Collaborative Study. BJU Int. 2016.
- Cheng JX, Tretiakova M, Gong C, Mandal S, Krausz T, et al. Renal medullary carcinoma: rhabdoid features and the absence of INI1 expression as markers of aggressive behavior. Mod Pathol. 2008;21:647–52.View ArticlePubMedGoogle Scholar
- Calderaro J, Moroch J, Pierron G, Pedeutour F, Grison C, et al. SMARCB1/INI1 inactivation in renal medullary carcinoma. Histopathology. 2012;61:428–35.View ArticlePubMedGoogle Scholar
- Calderaro J, Masliah-Planchon J, Richer W, Maillot L, Maille P, et al. Balanced translocations disrupting SMARCB1 Are hallmark recurrent genetic alterations in renal medullary carcinomas. Eur Urol. 2016;69:1055–61.View ArticlePubMedGoogle Scholar
- Ho TH, Swensen J, Ghazalpour A, Hes O, Stanton ML, Josehi M, et al. Comprehensive profiling of renal medullary and collecting duct carcinomas. J Clin Oncol. 2016;34:572. abstract.View ArticleGoogle Scholar
- Iacovelli R, Modica D, Palazzo A, Trenta P, Piesco G, et al. Clinical outcome and prognostic factors in renal medullary carcinoma: a pooled analysis from 18 years of medical literature. Can Urol Assoc J. 2015;9:E172–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Tannir NM, Plimack E, Ng C, Tamboli P, Bekele NB, et al. A phase 2 trial of sunitinib in patients with advanced non-clear cell renal cell carcinoma. Eur Urol. 2012;62:1013–9.View ArticlePubMedGoogle Scholar
- Lipkin JS, Rizvi SM, Gatalica Z, Sarwani NE, Holder SL, et al. Therapeutic approach guided by genetic alteration: use of MTOR inhibitor in renal medullary carcinoma with loss of PTEN expression. Cancer Biol Ther. 2015;16:28–33.View ArticlePubMedPubMed CentralGoogle Scholar
- Rathmell WK, Monk JP. High-dose-intensity MVAC for advanced renal medullary carcinoma: report of three cases and literature review. Urology. 2008;72:659–63.View ArticlePubMedGoogle Scholar
- Strouse JJ, Spevak M, Mack AK, Arceci RJ, Small D, et al. Significant responses to platinum-based chemotherapy in renal medullary carcinoma. Pediatr Blood Cancer. 2005;44:407–11.View ArticlePubMedGoogle Scholar
- Motzer RJ, Escudier B, McDermott DF, George S, Hammers HJ, et al. Nivolumab versus everolimus in advanced renal-cell carcinoma. N Engl J Med. 2015;373:1803–13.View ArticlePubMedGoogle Scholar
- Le DT, Uram JN, Wang H, Bartlett BR, Kemberling H, et al. PD-1 blockade in tumors with mismatch-repair deficiency. N Engl J Med. 2015;372:2509–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Rizvi NA, Hellmann MD, Snyder A, Kvistborg P, Makarov V, et al. Mutational landscape determines sensitivity to PD-1 blockade in non-small cell lung cancer. Science. 2015;348(6230):124–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Rosenberg JE, Hoffman-Censits J, Powles T, van der Heijden MS, Balar AV, et al. Atezolizumab in patients with locally advanced and metastatic urothelial carcinoma who have progressed following treatment with platinum-based chemotherapy: a single-arm, multicentre, phase 2 trial. Lancet. 2016;387(10031):1909–20.View ArticlePubMedGoogle Scholar
- Hugo W, Zaretsky JM, Sun L, Song C, Moreno BH, et al. Genomic and transcriptomic features of response to anti-PD-1 therapy in metastatic melanoma. Cell. 2016;165:35–44.View ArticlePubMedGoogle Scholar
- Heninger E, Krueger TE, Lang JM. Augmenting antitumor immune responses with epigenetic modifying agents. Front Immunol. 2015;6:29.PubMedPubMed CentralGoogle Scholar
- Yang H, Bueso-Ramos C, DiNardo C, Estecio MR, Davanlou M, et al. Expression of PD-L1, PD-L2, PD-1 and CTLA4 in myelodysplastic syndromes is enhanced by treatment with hypomethylating agents. Leukemia. 2014;28:1280–8.View ArticlePubMedGoogle Scholar
- Toor AA, Payne KK, Chung HM, Sabo RT, Hazlett AF, et al. Epigenetic induction of adaptive immune response in multiple myeloma: sequential azacitidine and lenalidomide generate cancer testis antigen-specific cellular immunity. Br J Haematol. 2012;158:700–11.View ArticlePubMedPubMed CentralGoogle Scholar
- Kovac M, Navas C, Horswell S, Salm M, Bardella C, et al. Recurrent chromosomal gains and heterogeneous driver mutations characterise papillary renal cancer evolution. Nat Commun. 2015;6:6336.View ArticlePubMedPubMed CentralGoogle Scholar
- Topalian SL, Hodi FS, Brahmer JR, Gettinger SN, Smith DC, et al. Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N Engl J Med. 2012;366:2443–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Tumeh PC, Harview CL, Yearley JH, Shintaku IP, Taylor EJ, et al. PD-1 blockade induces responses by inhibiting adaptive immune resistance. Nature. 2014;515:568–71.View ArticlePubMedPubMed CentralGoogle Scholar
- Daud AI, Loo K, Pauli ML, Sanchez-Rodriguez R, Sandoval PM, et al. Tumor immune profiling predicts response to anti-PD-1 therapy in human melanoma. J Clin Invest. 2016;126:3447–52.View ArticlePubMedPubMed CentralGoogle Scholar