Fig. 8
The down-modulation of CD19, CD20 and CD22 on Raji surface requires direct contact with CART cells. Multi-well plates with transwell inserts were used in this experiment. At the bottom of each well, 5 × 105 each of Raji and CAR T cells were combined, and in the transwell upper portion 2.5 × 105 Raji cells were cultured in the absence of T cells. After overnight incubation, cells from the upper transwell compartment and from the bottom compartment were harvested, and viable Raji cells were examined for CD19, CD20, and CD22 surface expression by flow cytometry (black, light grey, and dark grey bars, respectively. Surface expression for each marker with reference to the specific CAR T included in the lower compartment (x- axis) is shown. Bars depict mean + SD of three independent experiments performed using CAR T cells originating from three different donors. One way ANOVA, Dunnett’s multiple comparisons test *p < 0.05