Figure 2From: Presence of antigen-specific somatic allelic mutations and splice variants do not predict for immunological response to genetic vaccinationNative and splice variant mRNA transcripts of PAP are detectable in patients prior to vaccination. Panel A: Shown schematically are the four protein-coding transcripts of PAP including exons which are retained or excluded. Primer sets for ACPP-002 and ACPP-004 were designed to yield a native PAP product of indicated size as well as, if present, a variant PAP product of indicated size and as indicated by the gray boxes. Primer set for ACPP-003 was designed to yield a PAP-TM product of indicated size specific to the transmembrane domain-coding region of that transcript. Panel B: A representative agarose gel image of RTPCR products shows β-actin in lane 1, KLK3 (PSA) in lane 2, ACPP-002 native and variant bands in lane 3, ACPP-003 in lane 4, ACPP-004 native and variant bands in lane 5, no template control in lane 6, and 50 bp DNA ladder in lane 7.Back to article page