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Table 1 Human genes encoding common target antigens of genetic vaccines show previously detected allelic variations

From: Presence of antigen-specific somatic allelic mutations and splice variants do not predict for immunological response to genetic vaccination

Target antigen AR CEA gp100 MAGE-A3 NY-ESO-1 PAP PSA PSMA TYR
Gene ID AR-001 CEACAM5-001 PMEL-001 MAGEA3-001 CTAG1B-001 ACPP-001 KLK3-201 FOLH1-001 TYR-001
Location Xq12 19q13.1-13.2 12q13-q14 Xq28 Xq28 3q21-q23 19q13.41 11p11.2 11q14-q21
Synonymous Coding Mutations 26 28 22 16 0 13 20 19 24
Non-Synonymous Coding Mutations 71 61 46 43 1 22 39 38 95
Stop Gain/Loss 6 1 2 0 0 3 0 0 9
Frameshift 1 1 0 0 0 0 2 2 13
Coding Unknown 337 0 0 0 0 0 0 1 207
Total 441 91 70 59 1 38 61 60 348
cDNA Length (bp) 10065 2907 2757 1724 998 3125 1464 2635 2466
Protein Length (aa) 920 702 661 314 168 386 261 750 529
Mutation Quotient 0.085 0.090 0.073 0.137 0.006 0.065 0.157 0.053 0.221
  1. Eight genes encoding genetic vaccine target antigens under investigation in human clinical trials, and one gene encoding a prostate cancer target antigen of interest (AR), are listed in this table by antigen name as well as Gene ID taken from the USEast Ensemble online database. The NCBI-Gene and USEast Ensemble online databases provided information regarding chromosomal location of antigen gene, cDNA length (bp), and protein length (aa) as well as total number of allelic variations previously detected by others. Allelic variations are categorized into synonymous, non-synonymous, stop gain/loss, frameshift, or unknown coding mutations accordingly. Mutation quotient is defined as the sum of non-synonymous coding mutations, stop gain/loss, and frameshift mutations (mutations that could affect immunologically presented epitopes) divided by protein length (aa). Coding mutation online queries were performed April 16, 2012.