Presence of antigen-specific somatic allelic mutations and splice variants do not predict for immunological response to genetic vaccination

Table 4 Immune responses to peptides specific to PAP splice variant proteins are detectable in patients

		Library	Splice junction peptide pools					Control antigens
Patients		hPP	s2p1	s3p1	s3p2	s3p3	s4p1	rPP-100	SSX2-p167	PHA
	1			X	X		X			X
	2		X	X	X		X			X
	3				X					X
	4								X	X
	5									X
	6									X
Non-responders	7									X
	8									X
	10				X					X
	11				X					X
	12				X					X
	13									X
	14				X					X
	15	X			X					X
	16					X				X
	17								X	X
	18	X	X	X			X	X	X	X
	19								X	X
	24									X
Immune responders	25				X					X
	26				X					X
	27				X					X
	28				X					X
	29			X	X					X
	31				X					X
	32				X					X
	33									X

PBMC from immune non-responding patients (top, 1–13) and immune responding patients (bottom, 14–33) obtained prior to immunization were assessed for IFN-γ-secreting immune responses by ELISPOT following stimulation with peptides pools specific to the alternative splice variants of PAP or a peptide pool from the native antigen. The presence of a statistically significant IFN-γ-secreting immune response to a specific peptide pool as described above is indicated by an X.