CCR7, CD45RA, CD27 and CD28 subpopulations in tyrosinase
HLA*A0101-restricted tetramer-reactive CD8+T cells are consistent with an effector phenotype. PMBCs were analyzed by tetramer assay after in vitro culture with tyrosinase peptide pool. Representative dot plots for CD3+CD8+ T cells of patient Tyr-26 at Week 16 show the expression of CCR7, CD45RA, CD28 and CD27 on tyrosinase243-251 HLA*A0101 tetramer reactive CD8+ T cells (A). Further characterization in contour plots of CD3+CD8+tetramer+ T cells revealed that these cells were CCR7-CD45RA-CD27loCD28mid, consistent with an effector phenotype (B).