Figure 3

CCR7, CD45RA, CD27 and CD28 subpopulations in tyrosinase _243-251 HLA*A0101-restricted tetramer-reactive CD8⁺T cells are consistent with an effector phenotype. PMBCs were analyzed by tetramer assay after in vitro culture with tyrosinase peptide pool. Representative dot plots for CD3⁺CD8⁺ T cells of patient Tyr-26 at Week 16 show the expression of CCR7, CD45RA, CD28 and CD27 on tyrosinase_243-251 HLA*A0101 tetramer reactive CD8⁺ T cells (A). Further characterization in contour plots of CD3⁺CD8⁺tetramer⁺ T cells revealed that these cells were CCR7^-CD45RA^-CD27^loCD28^mid, consistent with an effector phenotype (B).