Figure 4

Increase in tyrosinase-reactive CD8⁺IFN-γ⁺T cells following mouse tyrosinase DNA vaccination. Intracellular cytokine staining was performed by multiparameter flow cytometry after culturing patient PBMCs with a tyrosinase peptide epitope pool for 10 days. These are representative dot plots of CD3⁺CD8⁺ T cells from Patient Tyr-25 who had an increase in CD8⁺IFN-γ⁺ cells following vaccination. There was evidence of polyfunctionality, including cells that were both IFN-γ⁺, and either CD107a⁺, MIP-1β⁺ or TNF-α⁺.