Figure 7
Mouse tyrosinase DNA vaccination and subsequent NY-ESO-1 specific tetramer and IFN-γ response. Patient Tyr-2 was known to have baseline seropositivity for NY-ESO-1. Thawed PBMCs from pre- and post- pINGmuTyr vaccination were cultured for 10 days with NY-ESO-1 overlapping peptides before tetramer analysis and ICS. Following vaccination, there was an increase in (A) HLA/B*35 NY-ESO-194-102 tetramer-reactive CD8+ T cells and (B) NY-ESO-1 specific CD4+ and CD8+ IFN-γ+ T cells.