T cell responses to STAT-5 are associated with overall survival in glioblastoma patients treated with DC vaccination. PBMC (pre/post DC vaccination) were either left unstimulated or stimulated with IL-2 for 20 minutes, followed by phospho-flow cytometry. pSTAT-5 histograms were gated from CD3+CD8+ T cells. Representative histograms from patients #34-730 (A) and #1-708 (B), document the pSTAT-5 expression in PBL samples when unstimulated, or stimulated with 500 IU IL-2 (pre/post DC vaccination). Note the differences in the pattern of pSTAT-5 expression before and after DC vaccination.