Individual patient cytokine production pre and post vaccine. The cytokine profiles for patients treated with 6 melanoma helper peptide vaccine. Cytokines were measured from PBMC’s cultured for 2 days with the 6 melanoma helper peptide mixture. Cytokines are shown in pg/ml. Arm A (patients 1-9) received 200 mcg, Arm B (patients 10-15) received 400 mcg and Arm C (patients 16-23) received 800 mcg. Cytokine cultures were performed in triplicate. All cytokine levels shown are corrected for background and negative control. Axes were scaled to show relative change from baseline. The ranges for the vertical differ between plots based on absolute cytokine level. IL-4 and IL-10 are available as Additional file 1: Figure S6.