Cytokine production at different time points following helper peptide vaccination. Cytokine levels in 5-day supernatants are shown for 6 patients with proliferative responses to 6MHP vaccination whose PBMC were evaluated after stimulation with 6MHP. Th1 and Th2 cytokines are represented by stacked bars. The time in weeks after first vaccine is shown on the x-axis. The SIN was collected at day 22. The prior node for patient number 21 was collected prior to helper peptide vaccination and was analyzed with permission. It is included to illustrate the pre-vaccine cytokine milieu of the lymph nodes.