Figure 4From: A melanoma helper peptide vaccine increases Th1 cytokine production by leukocytes in peripheral blood and immunized lymph nodes Cytokine secretion in response to 6MHP stimulation. PBMC (week 5, left panels) and SIN lymphocytes (day 22, right panels) were stimulated with 6MHP after CFSE labeling, then tested for IL-5 (top panels) production or IFN-γ (bottom panels) production. Data are shown for cells from a representative patient (patient 8) gated on lymphocytes, CD3-positive, and CD8-negative. The cells secreting cytokine are represented in the left upper quadrant of each plot, with percentages shown for each quadrant. IFN-γ producing cells are 8-9 fold more prevalent than IL-5 secreting cells in both PBMC and SIN’s.Back to article page