Murine CARs targeting CSPG4. Transduction efficiencies by FACS was determined using goat α-mouse Fab staining (A) for CAR detection. Data shown is representative of FACS data from three different PBL donors. Cells were cocultured with melanoma tumor cell lines to determine effector cytokine release. IFN-γ release when transduced PBL were co-cultured with CSPG4-positive melanoma target lines (B). Mel888 and mel1300 = melanoma cell lines. Anti-CSPG4 CAR-transduced T cells activity was statistically significant (p < 0.05, student’s t-test) in all comparisons to GFP control transduced T cells with the exception of 225.28 s transduced T cells from donor 2 (p > 0.05).