Figure 5From: Multiple chimeric antigen receptors successfully target chondroitin sulfate proteoglycan 4 in several different cancer histologies and cancer stem cells PBL transduced with murine CARs targeting CSPG4 recognize tumor lines from multiple cancer histologies. All CARs shown use the second generation CD28.CD3ζ signaling domain. A1207 = glioblastoma multiforme cell line, Mill = mesothelioma, MDAMB231 = breast cancer cell lines, MgG-63 = osteosarcoma cell line, mel938 = melanoma cell lines, H1299A = non-small cell lung cancer line (negative control). Anti-CSPG4 CAR-transduced T cells activity was statistically significant (p < 0.05, student’s t-test) in all comparisons to GFP control transduced T cells versus H1299 cells from donor 4. Anti-CSPG4 CAR-transduced T cells activity was statistically significant (p < 0.05) in all comparisons of TP41.2 and 149.53 CAR transduced cells to GFP control transduced T cells versus H1299 cells from donor 5. There was no significant difference in activity of 225.28S CAR transduced cells to GFP control transduced T cells versus H1299 cells from donor 5 in cocultures with MgG-63 and mel938 cells (p > 0.05).Back to article page