CSPG4 CAR recognition of glioma stem cells. (A) PBL from 3 donors were transduced with anti-CSPG4 murine CAR 225.28S-CD28.CD3ζ and co-cultured with the indicated GSC lines. Shown is the resultant IFN-γ release (pg/ml) following overnight co-culture (values are the average of duplicate determinations). (B) CSPG4 CAR-transduced PBL were mixed with the indicated GSC lines at various effector to target ratios and cytolytic function determined (shown as percent specific lysis). Data are representative of duplicate determinations.