VSSP modulates Th differentiation and protects CTL responses in TB mice. Mice bearing MCA203 tumors were s.c. inoculated with three doses of VSSP, or left untreated. (A) Mice were also vaccinated with OVA protein mixed with polyI:C and in vivo CTL response was measured. Graph shows the specific lysis, as a percentage of the control (tumor-free mice). Data are represented as mean of three individual mice ± SD. Two experiments with similar results were done. (B) Splenocytes from MCA203 TB mice, treated or not with VSSP, were stimulated in vitro with BM-DCs, previously pulsed with tumor lysates obtained from MCA203 and 3LL-D122 tumor cell lines. Production of IL-4, IL-17 and IFN-γ was detected by intracellular staining after 96 h stimulation. Graphs indicate the percentage of cells producing each cytokine, within the CD4+ T cells gate, for six individual mice belonging to two different experiments. Statistical analyses were performed with ANOVA and Tukey’s tests (A) or Kruskal-Wallis and Dunn’s tests (B).