Effector functions of TILs are improved as a consequence of VSSP inoculation. MCA203 TB mice were inoculated with VSSP on days 11, 12 and 18. On day 22, the s.c. tumors were extracted and TILs were isolated. (A) Tumor-specific response of CD8+ TILs after stimulation for 72 h with the MCA203 tumor cell line, or MB16F10 negative control. IFN-γ production by 106 CD8+ TILs was evaluated with ELISPOT assay. (B) Non-specific stimulation of TILs with Con A mitogen was additionally performed and the IFN-γ production was also evaluated by ELISPOT assay. Graphs indicate the mean of the number of IFN-γ spots from one experiment representative of two (T: tumor and V: VSSP). Statistically significant differences were detected with Student’s t test.