VSSP inhibits tumor-mediated expansion of Tregs in mice with high percentages of MDSCs. Splenic CD4+CD25+Foxp3+ Tregs were analyzed by FACS in VSSP-inoculated MCA203 TB mice, in comparison to untreated TB mice or tumor-free mice. A group of tumor-free mice inoculated with VSSP was also studied. Tregs percentages are referred to the gate of CD4+ T cells. (A) Dot plot graphs show the staining of Tregs in two representative mice per group. (B) Data are expressed as mean ± SD of five individual mice per group. ANOVA and Tukey’s tests were applied for statistical comparison. Data are representative of two experiments with similar results.