Figure 5
Functional enhancement of Pmel T cell cytokine production. Restimulation of Pmel T cell splenocytes with cognate gp10025-33 peptide ex vivo for five hours. Representative plots indicate splenocytes restimulated 10 days following adoptive cell transfer and are gated from CD8+ Thy1.1+ Pmel T cells. Gates were set based on Pmel cells that lacked peptide stimulation for the 5 hour duration of the restimulation. CD107a staining, as a marker for degranulation, was achieved by addition of CD107a antibody just immediately prior to the restimulation period. Results described are representative of at least three experiments with similar results. Student’s t-tests were performed to determine statistical significance between groups.