Volume 2 Supplement 1

Abstracts of the Updates on Immunotherapy of Cancer and Immunoscore Symposium: Sidra Symposia Series

Open Access

Minor tumor infiltrating B cells opened a door to reveal and eliminate cancer initiating cells in metastatic melanomas

  • Beatrix Kotlan1, 5,
  • Gabriella Liszkay2,
  • Gyorgy Naszados3,
  • Judit Olasz4,
  • Szabolcs Horvath5,
  • Vanda Plotar5,
  • Andras Szollar6,
  • Istvan Nagy Vamosi6,
  • Akos Savolt6,
  • Laszlo Toth6,
  • Orsolya Csuka4,
  • Maria Godeny3,
  • Miklos Kasler7 and
  • Francesco M Marincola8
Journal for ImmunoTherapy of Cancer20142(Suppl 1):P4


Published: 24 February 2014


The theory and investigations on cancer stem cells (CSCs) have received growing attention, as these cells are responsible for the failure of cancer therapeutic strategies and the return of cancer.

Materials and methods

A complex tumorimmunological study on primary and metastatic cancerous tissue biopsies and peripheral blood of patients with malignant melanomas (n = 153) has been performed with ethical permission (ETT TUKEB 16462- 02/2010) and patients’ formal consent.


We developed a methodology to select cancer initiating cells from fresh cancer cell cultures of malignant melanomas. Characteristic cell growth pattern, spheroid forming, CSC markers, like CD133, Nestin, ABCB5, CD20 and unique GD3 ganglioside expression were defined by immunofluorescence assay. Additionally tumor infiltrating B cells’ sialilated glycosphingolipid binding was defined by antibody phage display and immunglobulin repertoire analysis. Real Time PCR gene expression studies were done to reveal molecular parameters of regulatory mechanisms.


We provide here a novel strategy to detect cancer initiating cells in metastatic melanomas by double labeling with anti CD20 antibodies and sialilated glycosphingolipid antigen specific immunglobulins. With antibody engineering present findings might be turned into a novel cancer therapeutic approach targeting cancer stem cells.



Harry J. Lloyd Charitable Trust Melanoma Research Award/ 2010.

Authors’ Affiliations

Molecular Immunology and Toxicology, National Institute of Oncology
Oncodermatology, National Institute of Oncology
Radiological Diagnostics, National Institute of Oncology
Pathogenetics, National Institute of Oncology
Surgical and Molecular Tumorpathology, National Institute of Oncology
Oncosurgery, National Institute of Oncology
Board of Directors, National Institute of Oncology
Research, Sidra Medical and Research Center


© Kotlan et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.