Volume 2 Supplement 2

Abstracts from the 1st Immunotherapy of Cancer Conference (ITOC1)

Open Access

P16. Differential susceptibility of human and mouse NK cells to malignant cell-induced abnormalities in autologous combinations: a potential mechanism for the NK cell-based immunotherapy efficacy

  • G Sconocchia1,
  • R Arriga2,
  • S Caratelli1,
  • A Coppola2,
  • GC Spagnoli3,
  • G Lanzilli1,
  • B Capuani2,
  • F Ferrelli2,
  • D Lauro2 and
  • S Ferrone4
Journal for ImmunoTherapy of Cancer20142(Suppl 2):P7

https://doi.org/10.1186/2051-1426-2-S2-P7

Published: 12 March 2014

Background

Natural killer (NK) cells are highly effective in controlling tumour growth, in mice, but have no significant effect in humans. The reason(s) of this phenomenon is(are) unclear.

Methods

The effects of cancer cells on NK cells during target-effector cell conjugation was investigated utilising standard immunological methods including flow cytometry, chromium release and enzyme-linked immunosorbent assays while gene expression was evaluated by quantitative reverse transcriptase-polymerase chain reaction.

Results

We found that this phenomenon was associated with the different susceptibility of human and mouse NK cells to autologous tumour cell-induced NK cell abnormalities (NKCA). The latter includes CD16 down-regulation and NK cell depletion. Induction of NKCA by leukaemia and solid tumour cells was influenced neither by IL2 treatment nor by HLA class I antigen expression, but was abrogated by a 10 day culture. Following a 10 day of PBMCs culture, NK cells became resistant to leukaemia and solid tumor cell induced NKCA but maintained their cytotoxic activity. Actinomycin D restored the susceptibility of long term NK (LTNK) cells to NKCA suggesting that the generation of resistance to NKCA required RNA transcription. TAPI-0, a functional analogue of the tissue inhibitor of metalloproteinases (TIMP) 3 inhibited cancer cell induced NKCA underlying a role for a restricted number of metalloproteinases in the generation of this phenomenon. Finally, we found an association of TIMP3 gene and protein over-expression with the reduced susceptibility of LTNK cells to cancer cell induced NKCA.

Conclusions

This study provides evidence that TIMP3 plays a role in the protection of LTNK cells from cancer cell induced NKCA.

Authors’ Affiliations

(1)
Translational Pharmacology CNR, Biomedicine
(2)
Systems Medicine, University of Rome Tor Vergata
(3)
Surgical Research and Hospital Management, University of Basel, Biomedicine
(4)
Massachusetts General Hospital, Harvard Medical School, Surgery

Copyright

© Sconocchia et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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