Hepatic induction of IFN-λ genes during HCV infection. A, B. Total RNA was extracted from liver tissues of healthy and chronic HCV patients for IL-28A/B (A) and IL-29 (B) mRNA quantification by real-time PCR. C-F. Human primary hepatocytes were inoculated with UV-irradiated JFH-1 or JFH-1 for the indicated amounts of time, and IFN-λ gene levels were quantified by real-time PCR (C, E) while protein levels were analyzed by ELISA (D, F). Data represent means ± SD of three independent experiments (p<0.01).