Figure 4

Overexpression of IRFs and NF-κB confirms their role in expression of IFN-λ genes. PH5CH8 cells were transiently transfected with plasmids encoding different promoter regions of human IL-28A (pGL3-IL-28A 1kb) (A-C), IL-28B (pGL3-IL-28B 1kb) (D-F), or IL-29 (pGL3-IL-29 1kb) (G-J), and expression vectors encoding IRF-3, IRF-7, and NF-κB or a dominant-negative mutant of IKKβ (DNIκB). At 18 h post-transfection, cells were stimulated with poly(I:C) for 6 hours. Luciferase activity in whole cell lysates was normalized to Renilla luciferase activity. Data represent mean ± SD of triplicate data points of one experiment and are representative of five independent experiments (p<0.01).