TLR3, 7, 8, and 9 induce human IFN-λ gene expression in human primary hepatocytes. Human primary hepatocytes were incubated with JFH-1, Pam3CSK4 (1 μg/ml), HKLM (108 cells/ml), Poly (I-C) (5 μg/ml), LPS (10 μg/ml), Flagellin (1 μg/ml), FSL-1 (100 ng/ml), Imiquimod (1 μg/ml), ssRNA (1 μg/ml), or ODN2006 (5μM) for 24 hours. (A) Toll-like receptors (TLR) and their respective ligands. Supernatants were harvested and IL-28B (B) and IL-29 (C) were measured by ELISA. Data are the mean ± SD of triplicate data points from one experiment. Results are representative of three independent experiments. Statistical significance (p<0.01) is relative to JFH-1 infection.