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Figure 1 | Journal for ImmunoTherapy of Cancer

Figure 1

From: Imprime PGG, a yeast β-glucan immunomodulator, has the potential to repolarize human monocyte-derived M2 macrophages to M1 phenotype

Figure 1

M2 macophages derived from Imprimer PGG-treated monocytes have reduced expression of CD163, a key M2 marker. Human whole blood was treated with vehicle or Imprime PGG (25 μf/mL) for 2 hours at 37°C. CD14+ monocytes were then enriched using Ficoll gradient and magnetic bead separation. The cells (5 × 105 cells per mL) were then cultured in either M1-skewing (XVivo 10 media supplemented with 5% autologous serum and 50 ng/mL recombinant human GM-CSF) or M2-skewing (XVivo 10 media supplemented with 10% autologous serum and 100 ng/mL recombinant human M-CSF) conditions for 6 days. Macrophages were harvested and expression of a panel of M1/M2-specific markers was measured by flow cytometry. Shown here is a representative histogram of down-modulation of mean fluorescence intensity of CD163 marker in M" macrophages from four different experiments.

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