Figure 2

M2 macrophages derived from Imprime PGG-treated monocytes significantly enhance T-cell proliferation. M1 or M2 macrophages prepared as described in Figure 1 were cultured with CD3- and CD28-stimulated, CSFE-labeled, autologous CD4 T-cells at a 1:10 ratio for 3 days. T-cell proliferation was measured at the end of the experiement by flow cytometry, and results are graphically shown as CFSE-dilution peaks as well as quantitatively reported as division index (the average number of cell divisions a a population has undergone). Shown here is a representative assay with MS macrophages from three different experiments.