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  • Poster presentation
  • Open Access

Xeno-free serum replacement for ex vivo culture and expansion of T cells

  • 1,
  • 1,
  • 2 and
  • 1
Journal for ImmunoTherapy of Cancer20142 (Suppl 3) :P37

  • Published:


  • Human Serum
  • Cell Culture Medium
  • Transduction Efficiency
  • Cell Manufacturing
  • Serum Replacement


The manufacture of a majority of clinical T cell products for immunotherapy applications requires ex vivo T cell culture and expansion. Commercialization of T cell manufacturing processes requires reagents that meet regulatory guidelines and ultimately help reduce manufacturing cost of goods. A key component in many T cell culture protocols is human serum, which is expensive and may require testing prior to use for the manufacture of a cGMP-compliant T cell product. To this end, we have developed a xeno-free serum replacement supplement with defined components that can be used in combination with several different cell culture media to support ex vivo expansion of T cells.


T cells activated ex vivo and expanded with Dynabeads® CD3/CD28 CTSTM and cultured in OpTmizer™ CTS™, X-VivoTM 15, or AIM-V® CTS™ supplemented with pooled human serum or serum free T cell serum replacement showed similar growth kinetics, total fold expansion and transduction efficiency after 2 weeks in culture. Numbers of expanded CD4+ and CD8+ T cells were comparable in the expanded cultures regardless in the presence of human serum or the newly developed SRS-XF. Restimulated T cells expanded in serum free T cell serum replacement show similar cytokine profile and proliferation as T cells expanded in human serum.


This study shows that human serum may be replaced by a xeno-free formulation in several commonly used cell culture media to support ex vivo expansion and lentiviral transduction of polyclonal T cells. Culturing T cells in serum free T cell serum replacement facilitates a favourable culture profile and immune function. The serum free T cell serum replacement contains only fully tested human-derived or human recombinant proteins which facilitates supply security for clinical large scale and commercial therapies.

Authors’ Affiliations

Thermo Fisher Scientific, Oslo, Norway
Thermo Fisher Scientific, Grand Island, United States