Volume 3 Supplement 2

30th Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2015)

Open Access

Tumor-derived alpha-fetoprotein (tAFP) causes immune and metabolic dysfunction in monocyte-derived dendritic cells

  • Patricia M Santos1,
  • Angela Pardee1,
  • Greg M Delgoffe1 and
  • Lisa H Butterfield1
Journal for ImmunoTherapy of Cancer20153(Suppl 2):P210

https://doi.org/10.1186/2051-1426-3-S2-P210

Published: 4 November 2015

Background

Alpha-fetoprotein (AFP) is an oncofetal antigen expressed by over 50% of hepatocellular carcinoma (HCC) tumors. AFP-L3 is the major isoform present in the serum of HCC patients and is associated with poor patient prognosis. While HCC tumor-derived AFP (tAFP) contains >80% of AFP-L3, cord blood serum-derived AFP (nAFP) contains less than 5% of AFP-L3. Previous studies have proposed an immunoregulatory role for AFP on myeloid cells including dendritic cells (DC).

Methods

Therefore, to test the specific effect of nAFP and tAFP on DC differentiation in vitro, peripheral blood monocytes from healthy donors were cultured in the presence of nAFP or tAFP, and DC phenotype and function was assessed after 5 days. We have previously shown that monocytes cultured in vitro in the presence of tAFP differentiated into DC that retained a monocyte-like morphology, had decreased expression of surface DC maturation markers, exhibited limited production of inflammatory cytokines, and failed to induce robust T cell proliferative responses. Here, we investigate the mechanisms of tAFP-induced suppressive effects on monocyte-derived DC. Specifically: i) CD1 family surface expression, ii) chemokine production, and iii) DC metabolism.

Results

Our results show that 5 days after culture, the mRNA and surface expression of CD1a, CD1b, CD1c and CD1d are reduced in nAFP-DC and are further reduced in tAFP-DC. We also show that tAFP-DC had decreased secretion of chemokines CCL1, CCL2, CCL3, CCL4, CCL17, CCL20 and CCL22 in day 6 and/or day 7 supernatants on a per cell basis. Most importantly, we observe reduced mitochondrial mass and a significant defect in mitochondrial oxidative phosphorylation and inhibition of glycolysis in tAFP-DC compared to OVA-DC or nAFP-DC.

Conclusions

Collectively, these data show profound negative effects of tAFP on DC function. These results help explain some of the immune suppression observed in AFP+ HCC patients and may lead to novel therapeutic approaches to reverse these immunosuppressive effects to improve DC function and enhance anti-HCC immunity.

Authors’ Affiliations

(1)
University of Pittsburgh

Copyright

© Santos et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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