Volume 3 Supplement 2

30th Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2015)

Open Access

Induction of systemic and therapeutic antitumor immunity using intratumoral injection of bone-marrow derived dendritic cells genetically modified to express interleukin 12 combined with anti-CTLA-4 antibody

  • Marimo Sato-Matsushita1,
  • Yoshihiro Hayakawa2,
  • Asuka Asami1,
  • Setsuko Nakayama1 and
  • Hideaki Tahara1
Journal for ImmunoTherapy of Cancer20153(Suppl 2):P243

https://doi.org/10.1186/2051-1426-3-S2-P243

Published: 4 November 2015

We have been involved in the development of cancer immunotherapy using dendritic cells (DCs) manipulated to induce better immune responses. Our strategies include the usage of the agent to induce desirable maturation of DCs in culture and the genetic modification of DCs to have better function in situ. We have been developing immune-gene therapy with DCs genetically modified to continuously express IL-12. In this study, we investigated whether bone marrow-derived dendritic cells adenovirally transduced with genes encoding murine IL-12 combined with anti-CTLA-4 monoclonal antibody (Ad-IL-12-DCs) have significant therapeutic benefits for anti-tumor immunotherapy. Ad-IL-12-DCs was confirmed to express bioactive IL-12 proteins at high levels, and treatment with Ad-IL-12-DCs and anti-CTLA-4 monoclonal antibody showed enhanced anti-tumor effects and induced tumor-specific cytotoxic T lymphocyte responses in vivo. As a consequence of these stimulatory effects, combined treatment with DC vaccine and anti-CTLA-4 monoclonal antibody accomplished increased anti-tumor effects when compared with either mono-therapy. The information related to these trials would be useful to develop effective immunotherapy against cancer.

Authors’ Affiliations

(1)
The University of Tokyo
(2)
The University of Toyama

Copyright

© Sato-Matsushita et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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