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Figure 1 | Journal for ImmunoTherapy of Cancer

Figure 1

From: Common gamma chain (γc) cytokines differentially potentiate TNFR family signaling in antigen-activated CD8+ T cells

Figure 1

Influence of γc cytokines on proliferation, survival and activation state of recently-activated CD8 + T cells. Purified naïve OT-I CD8+ T cells (1x106/ml) were stimulated with peptide-pulsed APCs (6x106/ml). After 48 hours, OT-I T cells were harvested, re-purified and re-cultured (5x105/ml) in the presence of either plain media or media supplemented with IL-2, IL-4, IL-7, IL-15 or IL-21 (100 ng/ml). After 24 hours, cells were harvested and the analyzed. A) Expansion of OT-I CD8+ T cells in response to each treatment condition. B) Percentage of viable cells in each condition, as determined by Trypan Blue exclusion. C, D) Flow cytometry analysis of T cell markers associated with activation state, proliferation and exhaustion. Data are generated from 3 biological replicates, histograms reflect a single representative biological replicate and the bar graphs depict the mean of replicates +/-SD (n = 3). Unless otherwise noted, significance reflects the difference between the treatment group and the media only control. *P < 0.05, *P < 0.01, ***P < 0.001.

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