Figure 2

Effect of γc cytokines on the expression of co-stimulatory and co-repressive receptors by recently-activated CD8 ⁺ T cells. Purified naïve OT-I CD8⁺ T cells (1x10⁶/ml) were stimulated with peptide-pulsed APCs (6x10⁶/ml). After 48 hours, OT-I T cells were harvested, re-purified and re-cultured (5x10⁵/ml) in the presence of either plain media or media supplemented with IL-2, IL-4, IL-7, IL-15 or IL-21 (100 ng/ml). After 24 hours, cells were harvested and the analyzed by intracellular cytokine staining and flow cytometry. A, B) Expression of co-stimulatory CD8⁺ T cell receptors. C, D) Expression of co-repressive and suppressive CD8⁺ T cell receptors. Data are generated from 3 biological replicates, histograms reflect a single representative biological replicate and the bar graphs depict the mean of replicates +/-SD (n = 3). Unless otherwise noted, significance reflects the difference between the treatment group and the media only control. *P < 0.05, *P < 0.01, ***P < 0.001.