Figure 4

Differential changes in the functional polarization of CD8 ⁺ T cells induced by each γc cytokine. A-E) Genes are grouped into functional clusters reflecting distinct biological processes associated with CD8⁺ T cells. Functional clusters each contain multiple genes, which are represented as individual dots. Up-regulation (red) and down-regulation (blue) of gene expression are represented by color, and the amplitude of the change is represented by the size of the point. Values represent the mean of three biological replicates. Lines connecting genes represent putative gene-gene interactions, as defined by the Reactome FI database. Annotation of each cluster is based on a combination of a literature meta-study and the GO annotation database. Complete gene cluster, transcriptomic data and probe information is included in Additional file 3: Table S2. F) 3-dimensional bubble chart illustrating the distribution of all biological replicate from each experimental condition along three functional axes: pro-survival vs pro-apoptotic, peripheral homing vs central homing, and Tc1/Th1 vs Tc2/Th2 polarization.