Figure 5

Transcription heat maps for selected gene families associated with CD8 ⁺ T cell differentiation and functionality. Purified naïve OT-I CD8⁺ T cells (1x10⁶/ml) were stimulated with peptide-pulsed APCs (6x10⁶/ml). After 48 hours, OT-I T cells were harvested, re-purified and re-cultured (5x10⁵/ml) in the presence of either plain media or media supplemented with IL-2, IL-4, IL-7, IL-15 or IL-21 (100 ng/ml). After 24 hours, cells were harvested, mRNA isolated and gene expression analyzed by microarray. Data are generated from 3 biological replicates. Complete gene and probe information is included in Additional file 1: Table S1.