Patient 1 antigen-specific responses pre and post IMQ treatment. Ex-vivo MAGE and PRAME specific CD8+ (A) and CD4+ (B) T cell responses from bulk PBMCs of Patient 1 pre and post (post treatment and long term follow up 1 and 2) IMQ treatment were evaluated by intracellular cytokine staining for IFN-γ and TNF-α. CD8+ and CD4+ T cells were bead sorted from PBMCs collected post IMQ treatment and in vitro stimulated with CD8 and CD4 depleted PBMCs pulsed with MAGE or PRAME OLPs for 14-20 days. MAGE or PRAME specific CD8+ (C) and CD4+ (D) T cell responses were analyzed by intracellular cytokine staining for IFN-γ and TNF-α. Note that the flow plot for Prame looks different due to issues during acquisition of sample with too few cells. As controls, T cells were also stimulated with MOG and CEF OLPs and PMA/Ionomycin.