Patient 2 antigen-specific responses post IMQ treatment. Ex-vivo analysis of MAGE and PRAME specific CD8+ (A) and CD4+ (B) T cell responses from bulk PBMCs of Patient 2 by intracellular staining for IFN-γ and TNF-α. CD8+ and CD4+ T cells were bead sorted from PBMCs collected post IMQ treatment and in vitro stimulated with CD8 and CD4 depleted PBMCs pulsed with MAGE, PRAME, SURVIVIN, HER-2, or WT-1 OLPs for 14-21 days. Tumor antigen-specific CD8+ (C) and CD4+ (D) T cell responses were analyzed by intracellular staining for IFN-γ and TNF-α. As controls, T cells were also stimulated with MOG and CEF OLPs and PMA/Ionomycin.