Functional avidity of NY-ESO-1 specific T cells. A. NY-ESO-1 specific T cell products from patients #1-#6. The left panel for each patient shows the lysis of T2 cells pulsed with various concentrations of NY-ESO-1 peptide by NY-ESO-1-specific T cells at an effector to target (E:T) ratio of 20:1. The right panel shows lysis of the NY-ESO-1 expressing tumor cell line MelA375 at various E:T ratios. The tumor cell line Mel526 (NY-ESO-1 negative, gp100 positive) is a negative control. B. Lysis of NY-ESO-1 peptide pulsed T2 cells and tumor cells by a high affinity NY-ESO-1 T cell clone isolated by our lab, T cells transfected with the αLY TCR and sorted to >80% purity with NY-ESO-1 tetramer, and a gp100154-162 specific T cell clone.