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Table 2 Generation of clinical grade products

From: Tetramer guided, cell sorter assisted production of clinical grade autologous NY-ESO-1 specific CD8+ T cells

  Number of plates Number positive wells Least % positive sorted Largest % positive (all sorted) Mean % positive Number of wells used for expansion to final product Total cells after mini-REP (×10^6) Bag REP vs. GREX REP Cells started for large volume expansion (×10^6) Final cell number (×10^9)
Patient 1 3 3 0.62% 8.05% 3.39% 3 230 Bag 33 57.9
Patient 2 1 5 0.57% 1.90% 1.00% 5 73 GREX 68 44.2
Patient 3 1 7 0.01% 5.23% 1.23% 7 224 GREX 25 26.4
Patient 4 2 6 0.11% 3.68% 1.03% 4 157 GREX 19 20.7
Patient 5 2 3 0.07% 1.55% 0.57% 3 331 GREX 32  
Patient 6 2 5 0.11% 7.24% 2.80% 1 169 Bag 18 24.6
Mean 1.8 4.8 0.2% 4.6% 1.7% 3.8 197.3   32.4 34.8