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Table 2 Generation of clinical grade products

From: Tetramer guided, cell sorter assisted production of clinical grade autologous NY-ESO-1 specific CD8+ T cells

 

Number of plates

Number positive wells

Least % positive sorted

Largest % positive (all sorted)

Mean % positive

Number of wells used for expansion to final product

Total cells after mini-REP (×10^6)

Bag REP vs. GREX REP

Cells started for large volume expansion (×10^6)

Final cell number (×10^9)

Patient 1

3

3

0.62%

8.05%

3.39%

3

230

Bag

33

57.9

Patient 2

1

5

0.57%

1.90%

1.00%

5

73

GREX

68

44.2

Patient 3

1

7

0.01%

5.23%

1.23%

7

224

GREX

25

26.4

Patient 4

2

6

0.11%

3.68%

1.03%

4

157

GREX

19

20.7

Patient 5

2

3

0.07%

1.55%

0.57%

3

331

GREX

32

 

Patient 6

2

5

0.11%

7.24%

2.80%

1

169

Bag

18

24.6

Mean

1.8

4.8

0.2%

4.6%

1.7%

3.8

197.3

 

32.4

34.8