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Figure 3 | Journal for ImmunoTherapy of Cancer

Figure 3

From: Combined immune checkpoint protein blockade and low dose whole body irradiation as immunotherapy for myeloma

Figure 3

Blocking of PD-L1 in combination with TIM-3, LAG-3 or CTLA4 after lymphodepleting whole body irradiation synergistically improved survival. (A) Experimental design: KaLwRij mice received 500 cGy irradiation 7 days after tumor cell injection. Treatment with blocking antibody or control IgG (200 μg ip) was initiated 5 days later and specifically given 12, 14, 19, 21, 26, and 28 days after tumor injection. Survival curves of mice treated with (B) anti-TIM-3 only, or in combination with anti-PD-L1, (C) anti-LAG-3 only, or in combination with anti-PD-L1, (D) anti-CTLA4 only, or in combination with anti-PD-L1, (E) anti-CD48 only, or in combination with anti-PD-L1. Survival was compared with control antibody treated mice or mice treated with anti-PD-L1 only. Survival curves represent combined data from three (B, C, D) or two (E) independent experiments; n = 10-15 mice per experimental group. (F) Some of the survivors from panels B, C and D were re-challenged with 1×106 5T33 myeloma cells on day 110. P values were determined by the log-rank test.

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