The effector memory phenotype of T
cells transduced on day 3 and day 19. The phenotype of Day 3 (early) and Day 19 (late)-transduced VSTs was determined by flow cytometry after staining for CD3, CD45RO, CD62L, CCR7, CD8 and the GD2.CAR. (A) Gating of T cell memory subsets. CD3+GD2.CAR+ CD45RO+ cells were gated and their memory phenotype classified based on differential expression of CD62L and CCR7. Representative dot-plots from one donor out of six are shown (B). Percentages of CD62L+CCR7+ central memory cells, CD62L+CCR7− intermediate, CD62L−CCR7− effector memory T cells were gated as above in six donors. (C) The frequency of CD4+ and CD8+ cells in the CD3+GD2.CAR+ gate. The analysis of the phenotype was performed at the time of potential cryopreservation (day 11 for Day 3 transduced VSTs and day 26 for Day 19 transduced VSTs).